Identification of cardiac premature senescence markers through  GEO database interaction approach

Tiwuk Susantiningsih; Ani Retno Prijanti; Novi Silvia Hardiany; Fadilah

doi:10.32889/actabioina.110

Tiwuk Susantiningsih Department of Biochemistry Faculty of Medicine, UPN Veteran Jakarta, Jakarta, Indonesia https://orcid.org/0000-0002-5276-7997
Ani Retno Prijanti Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia https://orcid.org/0000-0003-0663-2714
Novi Silvia Hardiany Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia https://orcid.org/0000-0003-3359-5189
Fadilah Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia https://orcid.org/0000-0002-8120-3138

DOI: https://doi.org/10.32889/actabioina.110

Keywords: p53, database GEO, premature cardiac senescence, GEO database

Abstract

Background: Cardiomyocytes are cardiac muscle cells where premature senescence can occur. The availability of in silico research regarding premature senescence in cardiomyocytes is still limited.

Purpose: This in silico research aims to identify marker of cardiac premature senescence through the GEO database interaction approach.

Methods: This research used the GEO database with the GeneCards website approach using four keywords: premature senescence AND cardiovascular AND cardiomyocytes AND p53 followed by Cytoscape 3.9.1 analysis and StringDB analysis.

Results: From 1,046 proteins obtained, analyzed by using Cytoscape Tools series 3.9.1 resulted to 100 proteins having the highest score and continued with StringDB analysis with 16 proteins.

Conclusion: From the GeneCards, Cytoscape, and StringDB data, analysis of protein interactions in cardiac premature senescence showed that the protein-protein interactions with the highest scores were TP53, CDK2, and PTEN.

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References

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Identification of cardiac premature senescence markers through GEO database interaction approach

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