http://jurnal.pbbmi.org/index.php/actabioina <p>Acta Biochimica Indonesiana (ActaBiolna) [eISSN 2654-3222 / pISSN 2654-6108] is a peer-reviewed and open-access journal that disseminates original research articles and review articles covering diverse topics in Biochemistry and Molecular Biology. The journal is published biannually by Indonesian Society for Biochemistry and Molecular Biology.&nbsp;</p> Indonesian Society for Biochemistry and Molecular Biology en-US Acta Biochimica Indonesiana 2654-6108 http://jurnal.pbbmi.org/index.php/actabioina/article/view/135 <p><strong>Background:</strong> Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture.</p> <p><strong>Objective:</strong> This study aims to evaluate the capacity of ecoenzymes derived from local citrus organic waste to synthesize IAA hormones.</p> <p><strong>Methods:</strong> The ecoenzyme was extracted from fruit powders and centrifuged to separate the supernatant. One ml of ecoenzyme supernatant was then mixed with 2 ml of Salkowski reagent and incubated for 12 hours at room temperature in the dark to facilitate reaction. The presence and concentration of IAA were determined using spectrophotometry at a wavelength of 530 nm, while total protein levels were measured using the Warburg-Christian method.</p> <p><strong>Results:</strong> ecoenzymes from local citrus sources contain IAA, with the highest concentration observed in sample 7A (30.26 µg/ml). The ecoenzyme exhibited favorable characteristics, including an average degree of acidity of 3.55, and the highest total protein content was found in sample 2A (144.277 mg/mL).</p> <p><strong>Conclusion:</strong> Ecoenzymes from local orange peels successfully produce IAA, supported by fermentation-induced microbial activity and acidic conditions. This highlights their potential in sustainable agriculture.</p> Siska Alicia Farma Nurfa Dewiza Luzik Salma Sakina Irma Leilani Eka Putri Linda Advinda Azwir Anhar Copyright (c) 2023 Authors https://creativecommons.org/licenses/by-nc/4.0 2023-12-31 2023-12-31 6 2 135 135 10.32889/actabiona.137 http://jurnal.pbbmi.org/index.php/actabioina/article/view/137 <p><strong><span data-preserver-spaces="true">Background:&nbsp;</span></strong><span data-preserver-spaces="true">Diabetes mellitus is the most common endocrine disease globally. Hyperglycemia in diabetes mellitus is known to trigger oxidative stress. The activity of glutathione peroxidase (GSH-Px) is decreased in diabetic conditions. Catalase activity acts as a defense system to prevent the formation of free radicals (oxidants) in the human body.</span></p> <p><strong><span data-preserver-spaces="true">Objective:&nbsp;</span></strong><span data-preserver-spaces="true">This study aimed to determine the effectiveness of olive oil antioxidants on catalase activity and GSH-Px levels in hyperglycemic rats.</span></p> <p><strong><span data-preserver-spaces="true">Methods:&nbsp;</span></strong><span data-preserver-spaces="true">Adult male Wistar rats were randomized into three groups (n: 8): untreated control (Control), alloxan-induced untreated (Alloxan), and alloxan-induced treatment (Treatment) of olive oil at 25 mL/day for 14 days. Catalase activities were measured using the spectrophotometry method, and GSH-Px levels were analyzed using an enzyme-linked immunosorbent assay (ELISA).&nbsp;</span></p> <p><strong><span data-preserver-spaces="true">Results:</span></strong><span data-preserver-spaces="true">&nbsp;Administration of olive oil increased catalase activity compared to the untreated group in alloxan-induced rats. The average level of glutathione peroxidase was higher in the treatment group than in alloxan-induced rats.</span></p> <p><strong><span data-preserver-spaces="true">Conclusion:&nbsp;</span></strong><span data-preserver-spaces="true">Antioxidants in olive oil increased catalase activity and glutathione peroxidase levels in alloxan-induced hyperglycemic rats.</span></p> Ariani Zaltin Okvenda Eti Yerizel Raveinal Copyright (c) 2023 Authors https://creativecommons.org/licenses/by-nc/4.0 2023-12-31 2023-12-31 6 2 137 137