Enhancing cognitive function of healthy Wistar rats with aqueous extract of Centella asiatica

  • Ayeshah A Rosdah Department of Pharmacology, Faculty of Medicine, Universitas Sriwijaya, Palembang
  • Evi Lusiana Department of Pharmacology, Faculty of Medicine, Universitas Sriwijaya, Palembang
  • Muhammad Reagan Department of Internal Medicine, Faculty of Medicine, Universitas Sriwijaya, Palembang
  • Abdurrahman Akib Undergraduate Student, Faculty of Medicine, Universitas Sriwijaya, Palembang
  • Fadhila Khairunnisa Undergraduate Student, Faculty of Medicine, Universitas Sriwijaya, Palembang
  • Afkara Husna Undergraduate Student, Faculty of Medicine, Universitas Sriwijaya, Palembang
Keywords: Centella asiatica, Acetylcholine, Novel object recognition, Cognitive function, Cognitive performance


Background: Centella asiatica (L.) Urb is a native herb from Asian countries such as India, China, and Indonesia. This herb has been widely used as a cure for various diseases. However, studies investigating the aqueous extract of Centella asiatica as a nootropic in healthy individuals are still very limited.

Objective: This study aims to investigate the potential of aqueous extract of Centella asiatica in enhancing cognitive function of healthy male Wistar rats.

Methods: Rats were randomly allocated to four treatment groups, i.e. without treatment and aqueous Centella asiatica extract at doses of 200, 400 and 800 mg/kg. To determine enhancement of cognitive function, novel object recognition (NOR) test was conducted after the course of treatment. Acetylcholine content was assessed by enzyme-linked immunosorbent assay.

Results: There was a significantly high preference index towards the novel object in the NOR test in groups treated with 200 mg/kg and 800 mg/kg of the aqueous extract compared to control. This was further confirmed by a significant increase of brain acetylcholine content in rats treated with 200 mg/kg of the extract.

Conclusion: Therefore, this study confirms that the aqueous extract is effective in enhancing cognitive performance of healthy Wistar rats.


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How to Cite
Rosdah, A. A., Lusiana, E., Reagan, M., Akib, A., Khairunnisa, F., & Husna, A. (2018). Enhancing cognitive function of healthy Wistar rats with aqueous extract of Centella asiatica. Acta Biochimica Indonesiana, 1(2), 37-45. https://doi.org/10.32889/actabioina.v1i2.15